Regerend AMA kampioen Ben Spies was aan het eind van de tweede dag de snelste man op het befaamde Daytona. Mat Mladin, de snelste man van de eerste dag gaf er al snel de brui aan.
De tijden van de tweede testdag op Daytona
| p | naam | team | merk | tijd | klasse |
| 1 | Ben Spies | Yoshimura Suzuki | Suzuki GSX-R1000 | 1'39.016 | Superbike |
| 2 | Mat Mladin | Yoshimura Suzuki | Suzuki GSX-R1000 | 1'39.067 | Superbike |
| 3 | Jason DiSalvo | Graves Yamaha | Yamaha YZF-R1 | 1'39.274 | Superbike |
| 4 | Roger Hayden | Kawasaki | Kawasaki ZX-10R | 1'39.404 | Superbike |
| 5 | Jake Zemke | American Honda | Honda CBR1000RR | 1'39.467 | Superbike |
| 6 | Josh Hayes | Erion Honda | Honda CBR1000RR | 1'39.62 | Superbike |
| 7 | Miguel Duhamel | American Honda | Honda CBR1000RR | 1'40.067 | Superbike |
| 8 | Eric Bostrom | Graves Yamaha | Yamaha YZF-R1 | 1'40.080 | Superbike |
| 9 | Aaron Yates | Jordan Suzuki | Suzuki GSX-R1000 | 1'40.304 | Superstock |
| 10 | Akira Yanagawa | Kawasaki | Kawasaki ZX-10R | 1'40.461 | Superbike |
| 11 | Jamie Hacking | Kawasaki | Kawasaki ZX-10R | 1'41.639 | Superbike |
| 12 | Ben Bostrom | Graves Yamaha | Yamaha YZF-R1 | 1'41.775 | Superstock |
| 13 | James Ellison | Corona Honda | Honda CBR1000RR | 1'42.603 | Superbike |
| 18 | Josh Herrin | Graves Yamaha | Yamaha YZF-R6 | 1'44.76 | Supersport |
| 14 | Ryan Andrews | Rockwall Honda | Honda CBR600RR | 1'43.481 | Formula Xtreme |
| 15 | Aaron Gobert | Erion Honda | Honda CBR600RR | 1'43.804 | Formula Xtreme |
| 16 | Gary Mason | Corona Honda | Honda CBR1000RR | 1'44.007 | Superbike |
| 17 | Chaz Davies | Celtic Racing | Yamaha YZF-R6 | 1'44.565 | Supersport |
| 19 | Ryan Elleby | Rockwall Honda | Honda CBR600RR | 1'46.833 | Formula Xtreme |
